In Vitro Evaluation of the Antiinflammatory and Anticancer Activities of Compounds from the Plants Casearia sylvestris and Zanthoxylum monophyllum

• Omar A. Dupuy L.

  Instituto de Investigaciones en Biotecnología y Ciencias Biomédicas, Escuela de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panamá, Panamá

• José A. Bonilla V.

  Centro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, San José, Costa Rica

• Renato Murillo M.

  Escuela de Química y CIPRONA, Universidad de Costa Rica, San José, Costa Rica

• Peter Taylor

  Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Apartado 21827, Caracas 1020-A, Venezuela

• María J. Abad

  Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Apartado 21827, Caracas 1020-A, Venezuela

• Lorena I. González P.

  Instituto de Investigaciones en Biotecnología y Ciencias Biomédicas, Escuela de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panamá, Panamá

• Olmedo Otero

  Instituto de Investigaciones en Biotecnología y Ciencias Biomédicas, Escuela de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panamá, Panamá

• Johanna Juliao A.

  Instituto de Investigaciones en Biotecnología y Ciencias Biomédicas, Escuela de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panamá, Panamá

The anthocyanins, coumarins, and casearins are interesting compounds due to their chemical and biological properties. In the present study, the in vitro effect of 4 ́-O-methyl-gallocatechin (an anthocyanin), columbianatin (a coumarin), and casearin G on the mitogenic response and phagocytic activity from whole blood cells, was evaluated. In addition, the cytotoxic effect of these compounds on the cell lines B16/BL6 (murine melanoma) and COS-7 (kidney fibroblasts transfected with SV40 virus) was measured by a colorimetric assay (MTS/PMS, IC50: inhibitory concentration) and the sulphorhodamine B assay (GI50: growth inhibition, TGI: total growth inhibition, LC50: lethal concentration). The results show that 4 ́-O-methyl-gallocatechin and columbianatin reduced lymphoproliferation. Columbianatin reduced both the phagocytic index and the percentage of phagocytic monocytes/macrophages. Casearin G showed both cytotoxic (IC50, LC50) and cytostatic (GI50) effects against the tumor cells, B16/BL6 (IC50 = 30.8 μM; GI50 = 12.4 μM; LC50 = 34.7 μM) and COS-7 (IC50 = 137.3 μM; GI50 = 3.8 μM; LC50 = 29.9 μM). In conclusion, 4 ́-O-methyl-gallocatechin and columbianatin showed immunosuppressive properties in vitro while casearin G was the most cytotoxic.

Borges MH, Soares AM, Rodrigues VM, et al. Effects of aqueous extract of Casearia sylvestris (Flacourtiaceae) on actions of snake and bee venoms and on activity of phospholipases A2. Comp Biochem Physiol B Biochem Mol Biol 2000; 127: 21-30. http://dx.doi.org/10.1016/S0305-0491(00)00237-6

Oberlies NH, Burgess JP, Navarro HA, et al. Novel bioactive clerodane diterpenoids from the leaves and twigs of Casearia sylvestris. J Nat Prod 2002; 65: 95-9. http://dx.doi.org/10.1021/np010459m

Ferreira PM, Costa-Lotufo LV, Moraes MO, et al. Folk uses and pharmacological properties of Casearia sylvestris: a medicinal review. An Acad Bras Cienc 2011; 83(4): 1373-84. http://dx.doi.org/10.1590/S0001-37652011005000040

Bou DD, Lago JH, Figueiredo CR, et al. Chemical composition and cytotoxicity evaluation of essential oil from leaves of Casearia sylvestris, its main compound α-zingiberene and derivatives. Molecules 2013; 18: 9477-87. http://dx.doi.org/10.3390/molecules18089477

Dupuy OA, Murillo R, Bonilla JA. Actividad supresora del millerenólido sobre células mononucleares de sangre periférica humana. Rev Med Chil 2008; 136: 64-72. http://dx.doi.org/10.4067/S0034-98872008000100008

Dupuy OA, Murillo R, Bonilla JA. Lactonas sesquiterpénicas de las plantas Viguiera sylvatica y Decachaeta thieleana (Asteraceae) modulan la producción de óxido nítrico y la fagocitosis de macrófagos RAW. Rev Biol Trop 2008; 56: 1063-73.

Taylor PG, Dupuy OA, Bonilla JA, Murillo R. Anticancer activities of two sesquiterpene lactones, millerenolide and thieleanin isolated from Viguiera sylvatica and Decachaeta thieleana. Fitoterapia 2008; 79: 428-32. http://dx.doi.org/10.1016/j.fitote.2007.07.019

Rodríguez-Guzmán R, Fulks LC, Radwan MM, Burandt CL, Ross SA. Chemical constituents, antimicrobial and antimalarial activities of Zanthoxylum monophyllum. Planta Med 2011; 77: 1542-44. Supporting information for this article is available online at https://www.thieme-connect.de/media/plantamedica/201113/supmat/pmleu1147sup_10-1055-s-0030-1270782.pdf

Li X, Li Y, States VA, Li S, Zhang X, Martin RC. The effect of black raspberry extracts on MnSOD activity in protection against concanavalin A induced liver injury. Nutr Cancer 2014; 66(6): 930-7. http://dx.doi.org/10.1080/01635581.2014.922201

Toledo de Oliveira T, Nagem TJ, Rocha da Costa M, et al. Propiedades biológicas de los tintes naturales. Ars Pharmaceutica 2004; 45: 5-20.

Fylaktakidou KC, Hadjipavlou-Litina DJ, Litinas KE, Nicolaides DN. Natural and synthetic coumarin derivatives with anti-inflammatory/ antioxidant activities. Curr Pharm Des 2004; 10: 3813-33. http://dx.doi.org/10.2174/1381612043382710

Ahmad R, Asad M, Siddiqui ZN, Kumar A. Screening of synthetic new heterocyclic derivatives of 3-Formyl-4-Hydroxycoumarin for anti-inflammatory activity in albino rats. JPRHC 2009; 1: 46-62.

Menghini L, Epifano F, Genovese S, Marcotullio MC, Sosa S, Tubaro A. Antiinflammatory activity of coumarins from Ligusticum lucidum Mill. subsp. cuneifolium (Guss.) Tammaro (Apiaceae). Phytother Res 2010; 24: 1697-99. http://dx.doi.org/10.1002/ptr.3170

Sertié JA, Carvalho JC, Panizza S. Antiulcer activity of crude extracts from leaves of Casearia sylvestris. Pharm Biol 2000; 38: 112-9. http://dx.doi.org/10.1076/1388-0209(200004)3821-1FT112

Carvalho PR, Furlan M, Young MC, Kingston DG, Bolzani VS. Acetylated DNA-damaging clerodane diterpenes from Casearia sylvestris. Phytochemistry 1998; 49: 1659-62.

Dupuy OA, Bonilla JA, Murillo R, et al. Efecto in vitro de los terpenos lupeol y casearina G sobre células sanguíneas y tumorales. Rev Med Chil 2013; 141: 1150-57. http://dx.doi.org/10.4067/S0034-98872013000900007

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63. http://dx.doi.org/10.1016/0022-1759(83)90303-4

Bonilla JA, Mesén MG, Cartín W. Modificación de un método colorimétrico que usa XTT, para la determinación de linfoproliferación. Rev Cost Cienc Med 1993; 14: 25-31.

Skehan P, Storeng R, Scudiero D, et al. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst 1990; 82: 1107-12. http://dx.doi.org/10.1093/jnci/82.13.1107

Dongmo PMJ, Tchoumbougnang F, Sonwa ET, Kenfack SM, Zollo PHA, Menut C. Antioxidant and anti-inflammatory potential of essential oils of some Zanthoxylum (Rutaceae) of Cameroon. Int J Essential Oil Ther 2008; 2: 82-8.

Díaz W, Ortega F. Inventario de recursos botánicos útiles y potenciales de la cuenca del río Morón, estado Carabobo, Venezuela. Ernstia 2006; 16: 31-67.

Guzik TJ, Korbut R, Adamek-Guzik T. Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Pharmacol 2003; 54: 469-87.

Bor JY, Chen HY, Yen GC. Evaluation of antioxidant activity and inhibitory effect on nitric oxide production of some common vegetables. J Agric Food Chem 2006; 54: 1680-6. http://dx.doi.org/10.1021/jf0527448

Da Silva SL, Chaar J, Figueiredo P, Yano T. Cytotoxic evaluation of essential oil from Casearia sylvestris Sw on human cancer cells and erythrocytes. Acta Amaz 2008; 38: 107-12. http://dx.doi.org/10.1590/S0044-59672008000100012

Ameni A, Latorre A, Torres L, Górniak S. Risk assessment of medicinal plant Casearia sylvestris Sw (Salicaceae). Toxicol Lett 2014; 229: S129. http://dx.doi.org/10.1016/j.toxlet.2014.06.459

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