Anti-Cancer and Anti-Microbial Activity Studies of Some Complexes of Trimethoprim


  • Ali El-Shekeil Department of Chemistry, Faculty of Science, Sana’a University, P.O. Box 12463, Sana’a, Yemen
  • Abeer Omer Department of Chemistry, Faculty of Science, Sana’a University, P.O. Box 12463, Sana’a, Yemen
  • Sama Al-Aghbari Department of Chemistry, Faculty of Science, Sana’a University, P.O. Box 12463, Sana’a, Yemen
  • Omar Al-Shuja'a Department of Chemistry, Faculty of Applied Science, Thamar University, Thamar, Yemen



Trimethoprim complexes, anti-bacterial activity, anti-cancer activity.


 2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine, trimethoprim (TMP), was reacted with Cu, Zn, Fe and Ti. The products were characterized by elemental (CHN) analysis, FTIR, magnetic moment measurements, conductance measurements, electronic spectra, 13C spectra. All complexes were investigated for their anti-bacterial activities against Gram-positive and Gram-negative bacteria and were also screened for their in vitro anti-cancer potential using Hela and PC3 cells. All the complexes showed excellent anti-bacterial activity even more than TMP while TMP-Ti displayed good cytotoxic activity in vitro against Hela cells and TMP-Cu showed moderate cytotoxicity against PC3 cells.


Hitchings G, Burehall JJ, Nord F, Ed. Advances in Enzymology. Interscience, New York 1965; vol. 27: p. 417.

Louie A, Meade T. Metal complexes as enzyme inhibitors. J Chem Rev 1999; 99: 2711.

William R. The biochemistry of sodium, potassium, magnesium and calcium. Q Rev Chem Soc 1971; 24: 331.

Heim M. Platinum and non-platinum complexes In clinical trials Current status and new developments. Metal Complexes in Cancer Chemotherapy Verlag Chemie, Weinheim 1993.

Williams D. The metals of life: The solution chemistry of metal ions in biological systems Van Nostrand, London 1971.

William R. Bioinorganic Chemistry. American Chemical Society, Washington 1971.

Gillard R. Inorg Chim Acta Rev 1961; 1: 60.

Scozzafava A, Menavuoni L, Mincione F, Mincione G, Supuran C. Carbonic anhydrase inhibitors: synthesis of sulfonamides incorporating dtpa tails and of their zinc complexes with powerful topical antiglaucoma properties. Bioorg Med Chem Lett 2001; 11: 575.

Scozzafava A, Supuran C. Carbonic anhydrase and matrix metalloproteinase inhibitors: sulfonylated amino acid hydroxamates with MMP inhibitory properties act as efficient inhibitors of CA isozymes I, II, and IV, and N-hydroxysulfonamides inhibit both these zinc enzymes. J Med Chem 2000; 43: 3677.

Walsh C. Enabling the chemistry of life. Science 2001; 409: 226.

Bertien I, Gray H, Lippard S, Valentine J. Bio-inorganic chemistry. Univ Science Books, Mill Valey 1994.

Kovala-Demertzi D, Hadjiliadis N, Tsangaris JM, Less J. ‘Complexes Tf 2′,4′- diamino-5(3′,4′,5′-trimethoxy) pyrimidine(trimethroprim) with Pt (II). Rh(II) and Au(II) Common Metals 1986; 115: 1.

Tsangaris JM, Sotiropoulos D, Galinos AG. Complexes of trimethoprim with CuCl2, NiCl2 and CoCl2. Inorg Nucl Chem Lett 1978; 14: 375.

Demartin F, Manassero M, Naldini L, Zoroddu MA. Synthesis and crystal structure of CoCl2(TRIMETHOPRIM)2 Inorg. Chim Acta L 1983; 213: 77.

Naldini L, Cabras MA, Zoroddu MA, Demartin F, M. Man- 3 2 assero, M. Sansoni, Synthesis, magnetic characterization and X-ray structure of

Zoroddu MA, Naldini L, Demartin F, Masciocchi N. Syntheses and Xray Structures of

Demartin F, Manassero M, Naldini L, Panzanelli A, Zoroddu MA. Synthesis and X-Ray structure of

Muthiah PT, Robert JJ. synthesis and crystal structure of a cadmium complex of trimethoprim (

Seekhon BS, Randhawa HS, Sahai HK. On the coordination behavior of Trimethoprim towards some metal ions. Synth React Inorg Met-Org Chem 1999; 29(2): 309.

Habib U, Bashah A, Florke U, Rquresh RA, Mirza B, Islam NU, Khan A. Synthesis and Structural Characterization of (2,4Diamino5-(3′,4′,5′-Trimethoxybenzyl)Pyrimidine Copper (II)) Complex. J Chem Crystallog 2009; 39: 730-34.

Muanza DN, Kim BW, Euler KL, Williams L. Antibacterial and antifungal activities of nine medicinal plants from Zaire. Int J Pharmacogn 1994; 32: 337-45.

Irob ON, Moo-Young M, Anderson WA. Antimicrobial activity of Annato Extract. Intl J Pharmacogn 1996; 34: 87-90.

Mosmann TJ. Immunol Methods 1983; 65: 55-63.

Simo B, Perello L, Ortiz R, Castineiras A, Latorre J, Canton E. Interactions of metal ions with a 2,4-diaminopyrimidine derivative (trimethoprim). J Inorg BioChem 2000; 81: 275-83.




How to Cite

Ali El-Shekeil, Abeer Omer, Sama Al-Aghbari, & Omar Al-Shuja’a. (2013). Anti-Cancer and Anti-Microbial Activity Studies of Some Complexes of Trimethoprim . Journal of Cancer Research Updates, 2(1),  14–20.