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Non-Invasive Real-Time Monitoring and Label-Free Sensing of Hydrogen Peroxide in Human Brain Cancer Cells Through Raman Spectroscopy

Authors

  • Darrell B. Tata

    U.S. Food and Drug Administration, Center for Devices and Radiological Health, Office of Science and Engineering Laboratories, Silver Spring, MD 20993, USA

  • Ilko K. Ilev

    U.S. Food and Drug Administration, Center for Devices and Radiological Health, Office of Science and Engineering Laboratories, Silver Spring, MD 20993, USA

DOI:

https://doi.org/10.6000/1927-7229.2012.01.02.2

Keywords:

Raman spectroscopy, human glioblastoma, hydrogen peroxide, biomarkers, cellular metabolic activity

Abstract

 We present evidence that real-time optical monitoring of human brain cancer cells through forward Raman spectroscopy can be used as a highly sensitive non-invasive label-free tool in acquiring real-time information on the cellular metabolic activity through the concentration levels of selective biomarkers. In this investigation, we quantified the concentration level of a natural by-product of cellular respiration, hydrogen peroxide (H2O2), through its characteristic Raman signature due to the transitions of O-O vibration energy level, resulting in a sharp Raman scatter frequency shift around 880cm-1. The experimental findings revealed that 3.75´106 cells to have produced a substantial amount (3%) of H2O2.

References

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Published

2012-07-15

Issue

Vol. 1 No. 2 (2012)

Section

Articles

How to Cite

Non-Invasive Real-Time Monitoring and Label-Free Sensing of Hydrogen Peroxide in Human Brain Cancer Cells Through Raman Spectroscopy . (2012). Journal of Analytical Oncology, 1(2),  145-148. https://doi.org/10.6000/1927-7229.2012.01.02.2

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