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Curcumin Inhibits Retinoblastoma Cell Proliferation by miR-26a Targeting the Tumor Suppressor Gene Rb1 in Y79 Cells

Authors

  • Jing Bai

    Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China

  • Duo Guo

    Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University, Beijing, 100069, China

  • Xiangyi Liu

    Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730, China

DOI:

https://doi.org/10.30683/1927-7229.2020.09.08

Keywords:

Retinoblastoma, miR-26a, Rb1, microRNA, Curcumin.

Abstract

The retinoblastoma (Rb1) gene is one of the most important tumor suppressor genes. Dysfunction of Rb protein drives tumorigenesis by overcoming barriers to cellular proliferation. Consequently, factors modulating Rb function are of great clinical import. Here, we show that miR-26a was differentially expressed in human retinoblastoma cells, tissues and serums from retinoblastoma patients, compared with human retinal microvascular endothelial cells, non-tumor tissues and serums from healthy children, and that it tightly regulated the expression of Rb1 by specifically targeting a conserved sequence motif in its UTR, leading to low expression of Rb1. In vitro experiments determined that miR-26a directly participated in the regulation of cell proliferation of human Y79 RB cells. Our results also suggest that curcumin modulated the miR-26a expression profile, thereby exerting its anti-proliferation effects on Y79 RB cells via up-regulation of Rb1. To our knowledge, these data indicate for the first time that miR-26a directly regulates cell proliferation by targeting Rb1 in retinoblastoma and that miR-26a could be a potential therapeutic approach for retinoblastoma.

 

References

Vogel F. Genetics of retinoblastoma. Hum Genet 1979 1; 52(1): 1-54. https://doi.org/10.1007/BF00284597

Zhang J, Benavente CA, McEvoy J, Flores-Otero J, Ding L, Chen X, Ulyanov A, Wu G, Wilson M, Wang J, Brennan R, Rusch M, Manning AL, Ma J, Easton J, Shurtleff S, Mullighan C, Pounds S, Mukatira S, Gupta P, Neale G, Zhao D, Lu C, Fulton RS, Fulton LL, Hong X, Dooling DJ, Ochoa K, Naeve C, Dyson NJ, Mardis ER, Bahrami A, Ellison D, Wilson RK, Downing JR, Dyer MA. A novel retinoblastoma therapy from genomic and epigenetic analyses. Nature 2012; 481(7381): 329-34. https://doi.org/10.1038/nature10733

Sakai T1, Toguchida J, Ohtani N, Yandell DW, Rapaport JM, Dryja TP. Allele-specific hypermethylation of the retinoblastoma tumor-suppressor gene. Am J Hum Genet 1991; 48(5): 880-8.

Dalgard CL, Gonzalez M, deNiro JE, O'Brien JM. Differential microRNA-34a expression and tumor suppressor function in retinoblastoma cells. Invest Ophthalmol Vis Sci 2009; 50(10): 4542-51. https://doi.org/10.1167/iovs.09-3520

Filipowicz W, Bhattacharyya SN, Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat Rev Genet 2008; 9(2): 102-14. https://doi.org/10.1038/nrg2290

Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, Prueitt RL, Yanaihara N, Lanza G, Scarpa A, Vecchione A, Negrini M, Harris CC, Croce CM. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A 2006; 103(7): 2257-61. https://doi.org/10.1073/pnas.0510565103

Esquela-Kerscher A, Slack FJ. Oncomirs-microRNAs with a role in cancer, Nat Rev Cancer 2006; 6: 259-269. https://doi.org/10.1038/nrc1840

Zhao JJ, Yang J, Lin J, et al. Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis. Nat Rev Cancer 2006; 6(4): 259-69.

Park JK, Henry JC, Jiang J, Esau C, Gusev Y, Lerner MR, Postier RG, Brackett DJ, Schmittgen TD. miR-132 and miR-212 are increased in pancreatic cancer and target the retinoblastoma tumor suppressor. Biochem Biophys Res Commun 2011; 406(4): 518-23. https://doi.org/10.1016/j.bbrc.2011.02.065

Jo DH, Kim JH, Park WY, Kim KW, Yu YS, Kim JH. Differential profiles of microRNAs in retinoblastoma cell lines of different proliferation and adherence patterns. J Pediatr Hematol Oncol 2011; 33(7): 529-33. https://doi.org/10.1097/MPH.0b013e318228280a

Dalgard CL, Gonzalez M, deNiro JE, O'Brien JM. Differential microRNA-34a expression and tumor suppressor function in retinoblastoma cells. Invest Ophthalmol Vis Sci 2009; 50(10): 4542-51. https://doi.org/10.1167/iovs.09-3520

Kandalam MM, Beta M, Maheswari UK, Swaminathan S, Krishnakumar S. Oncogenic microRNA 17-92 cluster is regulated by epithelial cell adhesion molecule and could be a potential therapeutic target in retinoblastoma. Mol Vis 2012; 18: 2279-87.

Yu J, Cai X, He J, Zhao W, Wang Q, Liu B. Microarray-based analysis of gene regulation by transcription factors and microRNAs in glioma. Neurol Sci 2013; 34(8): 1283-9. https://doi.org/10.1007/s10072-012-1228-1

Guo D, Dong LY, Wu Y, Yang L, An W. Down-regulation of hepatic nuclear factor 4α on expression of human hepatic stimulator substance via its action on the proximal promoter in HepG2 cells. Biochem J 2008; 415(1): 111-21. https://doi.org/10.1042/BJ20080221

Sreenivasan S, Thirumalai K, Danda R, Krishnakumar S. Effect of curcumin on miRNA expression in human Y79 retinoblastoma cells. Curr Eye Res 2012; 37(5): 421-8. https://doi.org/10.3109/02713683.2011.647224

Lee EY, Bookstein R, Young LJ, Lin CJ, Rosenfeld MG, Lee WH. Molecular mechanism of retinoblastoma gene inactivation in retinoblastoma cell line Y79. Proc Natl Acad Sci USA 1988; 85(16): 6017-21. https://doi.org/10.1073/pnas.85.16.6017

Ciarapica R, Russo G, Verginelli F, Raimondi L, Donfrancesco A, Rota R, Giordano A. Deregulated expression of miR-26a and Ezh2 in rhabdomyosarcoma. Cell Cycle 2009; 8(1): 172-5. https://doi.org/10.4161/cc.8.1.7292

Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Mendell JR, Mendell JT. Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Cell 2009; 137(6): 1005-17. https://doi.org/10.1016/j.cell.2009.04.021

Chau BN, Wang JY. Coordinated regulation of life and death by RB. Nat Rev Cancer 2003; 3(2): 130-8. https://doi.org/10.1038/nrc993

Kanoe H, Nakayama T, Murakami H, Hosaka T, Yamamoto H, Nakashima Y, Tsuboyama T, Nakamura T, Sasaki MS, Toguchida J. Amplification of the CDK4 gene in sarcomas: tumor specificity and relationship with the RB gene mutation. Anticancer Res 1998; 18(4A): 2317-21.

Horowitz JM, Park SH, Bogenmann E, Cheng JC, Yandell DW, Kaye FJ, Minna JD, Dryja TP, Weinberg RA. Frequent inactivation of the retinoblastoma anti-oncogene is restricted to a subset of human tumor cells. Proc Natl Acad Sci USA 1990; 87(7): 2775-9. https://doi.org/10.1073/pnas.87.7.2775

Kim H, Huang W, Jiang X, Pennicooke B, Park PJ, Johnson MD. Integrative genome analysis reveals an oncomir/ oncogene cluster regulating lioblastoma Survivorship. Proc Natl Acad Sci USA 2010; 107(5): 2183-8. https://doi.org/10.1073/pnas.0909896107

Zhang YF, Zhang AR, Zhang BC, Rao ZG, Gao JF, Lv MH, Wu YY, Wang SM, Wang RQ, Fang DC.MiR-26a regulates cell cycle and anoikis of human esophageal adenocarcinoma cells through Rb1-E2F1 signaling pathway. Mol Biol Rep 2013; 40(2): 1711-20. https://doi.org/10.1007/s11033-012-2222-7

Sharma RA, Gescher AJ, Steward WP. Curcumin: the story so far. Eur J Cancer 2005; 41(13): 1955-68. https://doi.org/10.1016/j.ejca.2005.05.009

Mukhopadhyay A, Bueso-Ramos C, Chatterjee D, Pantazis P, Aggarwal BB. Curcumin downregulates cell survivalmechanisms in human prostate cancer cell lines. Oncogene 2001; 20(52): 7597-609. https://doi.org/10.1038/sj.onc.1204997

Rogoff HA, Kowalik TF.Life, death and E2F: linking proliferation control and DNA damage signaling via E2F1. Cell Cycle 2004; 3(7): 845-6. https://doi.org/10.4161/cc.3.7.975

Liu R, Zhang C, Hu Z, Li G, Wang C, Yang C, Huang D, Chen X, Zhang H, Zhuang R, Deng T, Liu H, Yin J, Wang S, Zen K, Ba Y, Zhang CY. A five-microRNA signature identified from genome-wide serum microRNA expression profiling serves as a fingerprint for gastric cancer diagnosis. Eur J Cancer 2011; 47(5): 784-91. https://doi.org/10.1016/j.ejca.2010.10.025

Tsujiura M, Ichikawa D, Komatsu S, Shiozaki A, Takeshita H, Kosuga T, Konishi H, Morimura R, Deguchi K, Fujiwara H, Okamoto K, Otsuji E. Circulating microRNAs in plasma of patients with gastric cancers. Br J Cancer 2010; 102(7): 1174-9. https://doi.org/10.1038/sj.bjc.6605608

Yamada Y, Enokida H, Kojima S, Kawakami K, Chiyomaru T, Tatarano S, Yoshino H, Kawahara K, Nishiyama K, Seki N, Nakagawa M. MiR-96 and miR-183 detection in urine serve as potential tumor markers of urothelial carcinoma: correlation with stage and grade, and comparison with urinary cytology. Cancer Sci 2011; 102(3): 522-9. https://doi.org/10.1111/j.1349-7006.2010.01816.x

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Published

2021-02-19

Issue

Vol. 9 (2020)

Section

Articles

How to Cite

Curcumin Inhibits Retinoblastoma Cell Proliferation by miR-26a Targeting the Tumor Suppressor Gene Rb1 in Y79 Cells . (2021). Journal of Analytical Oncology, 9, 63-71. https://doi.org/10.30683/1927-7229.2020.09.08

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