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RNA-Sequencing-Based lncRNA Biomarker Profiling on Triple Negative Breast Cancer

Authors

  • Lin Gao

    Department of Clinical Medical Research Center, The Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital); The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518000, Guangdong, P.R. China

  • Qinhe Yang

    Department of Integrated Chinese and Western Medicine, Jinan University, Guangzhou, Guangdong, P.R. China

  • Wenbin Zhou

    Department of Thyroid and Breast Surgery, The Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital); The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518000, Guangdong, P.R. China

  • Yong Dai

    Department of Clinical Medical Research Center, The Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital); The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518000, Guangdong, P.R. China

  • Jigang Wang

    Department of Clinical Medical Research Center, The Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital); The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518000, Guangdong, P.R. China

  • Li Fu

    Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Department of Pharmacology and International Cancer Center, Shenzhen University Health Science Center, Shenzhen 518060, Guangdong, China

  • Pan Zhao

    Shenzhen Public Service Platform on Tumor Precision Medicine and Molecular Diagnosis, the Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital), Shenzhen, Guangdong, PR China

  • Jingy Huang

    Department of Clinical Medical Research Center, The Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital); The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518000, Guangdong, P.R. China

  • Malin Hong

    Department of Clinical Medical Research Center, The Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital); The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518000, Guangdong, P.R. China

  • Jinquan Xia

    Department of Clinical Medical Research Center, The Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital); The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518000, Guangdong, P.R. China

  • Chang Zou

    Shenzhen Public Service Platform on Tumor Precision Medicine and Molecular Diagnosis, the Second Clinical Medical College, Jinan University (Shenzhen People’s Hospital), Shenzhen, Guangdong, PR China

DOI:

https://doi.org/10.30683/1929-2279.2021.10.01

Keywords:

TNBC, long noncoding RNA (lncRNA), RNA-sequencing, ZEB1-AS1, TMEM254-AS1, LINC01087, LINC01122, LINC00856.

Abstract

Abstract: Basal-like triple-negative breast cancer (TNBC) is highly heterogeneous and lack of effective molecular targets for therapy. In this study, we developed the lncRNA signatures of TNBC as molecular biomarkers. RNA-sequencing in 12 paired breast cancer and adjacent tissues identified up-regulated and down-regulated lncRNAs of Basal subtype in contrast to Luminal A, Luminal B and HER2 subtypes. Additionally, Kaplan-Meier analysis revealed that high expression of lncRNA (ZEB1-AS1 and TMEM254-AS1) had a poor relapse-free survival rate (RFS), while high expression of lncRNA (LINC01087, LINC01122 and LINC00856) had a positive correlation with RFS. Furthermore, qRT-PCR analysis showed that the mRNA expressions of the ZEB1-AS1 and TMEM254-AS1 lncRNA were up-regulated in TNBC tissues, while the mRNA expression of lncRNA, including LINC01087, LINC01122 and LINC00856 were down-regulated in TNBC tissues. Taken together, our results elucidated that 5 novel lncRNAs, including ZEB1-AS1, TMEM254-AS1, LINC01087, LINC01122 and LINC00856 contributed to the progression of invasive TNBC. These lncRNAs could be molecular biomarkers for the development of TNBC treatment.

Keywords: TNBC, long noncoding RNA (lncRNA), RNA-sequencing, ZEB1-AS1, TMEM254-AS1, LINC01087, LINC01122, LINC00856.

References

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Published

2021-05-27 — Updated on 2021-05-27

Issue

Vol. 10 (2021)

Section

Articles

How to Cite

RNA-Sequencing-Based lncRNA Biomarker Profiling on Triple Negative Breast Cancer. (2021). Journal of Cancer Research Updates, 10, 1-9. https://doi.org/10.30683/1929-2279.2021.10.01

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