Short Communication: Studying the Role of Smart Flare Gold Nano Particles in Studying Micro RNA and Oncogene Differential Expression in Prostate Cancer Cell Lines
DOI:
https://doi.org/10.6000/1929-2279.2017.06.02.1Keywords:
Nanoparticles, micro RNA, gene expression.Abstract
Nano technology is a cutting edge science which is now effectively used in the field of cancer biology. Smart Flare gold nanoparticles are now used often for differential gene expression analysis. In this manuscript we are reporting the use of micro RNA miR 146a and onco gene EZH2 Smart Flare probes to study their expression in different prostate cancer cell lines and the effect of novel Rhenium compounds on these genes using a flow cytometer and a Fluorescence microscope. Our results showed this novel nanotechnology can be effectively used in cancer biology to successfully detect the effect of novel drugs on oncogenes and could be a very useful tool for next generation of cancer researchers.
References
Liu Z, Xiao B, Tang B, Li B, Li N, Zhu E, et al. Up-regulated microRNA-146a negatively modulate Helicobacter pylori-induced inflammatory response in human gastric epithelial cells. Microbes Infect 2010; 12(11): 854-63. https://doi.org/10.1016/j.micinf.2010.06.002
Sonkoly E, Stahle M, Pivarcsi A. MicroRNAs and immunity: novel players in the regulation of normal immune function and inflammation. Semin Cancer Biol 2008; 18(2): 131-40. https://doi.org/10.1016/j.semcancer.2008.01.005
Williams AE, Perry MM, Moschos SA, Larner-Svensson HM, Lindsay MA. Role of miRNA-146a in the regulation of the innate immune response and cancer. Biochem Soc Trans 2008; 36(6): 1211-15. https://doi.org/10.1042/BST0361211
Philippidou D, Schmitt M, Moser D, Margue C, Nazarov PV, Muller A, et al. Signatures of microRNAs and selected microRNA target genes in human melanoma. Cancer Res 2010; 70(10): 4163-73. https://doi.org/10.1158/0008-5472.CAN-09-4512
Zhong H, Wang HR, Yang S, Zhong JH, Wang T, Wang C, et al. Targeting Smad4 linksmicroRNA-146a to the TGF-beta pathway during retinoid acid induction in acute promyelocytic leukemia cell line. Int J Hematol 2010; 92(1): 129-35. https://doi.org/10.1007/s12185-010-0626-5
Wang LH, Kim SH, Lee JH, Choi YL, Kim YC, Park TS, et al. Inactivation of SMAD4 tumor suppressor gene during gastric carcinoma progression. Clin Cancer Res 2007; 13(1): 102-10. https://doi.org/10.1158/1078-0432.CCR-06-1467
He H, Jazdzewski K, Li W, Liyanarachchi S, Nagy R, Volinia S, et al. The role of microRNA genes in papillary thyroid carcinoma. Proc Natl Acad Sci USA 2005; 102(52): 19075-80. https://doi.org/10.1073/pnas.0509603102
Mbagu MK, Kebulu DN, Winstead A, Pramanik SK, Banerjee HN, Iwunze MO, et al. Fac-tricarbonyl(pentylcarbonato)(α-diimine)rhenium complexes: One-pot synthesis, characterization, fluorescence studies, and cytotoxic activity against human MDA-MB-231 breast, CCl-227 colon and BxPC-3 pancreatic carcinoma cell lines. Inorg Chem Commun 2012; 21: 35-38. https://doi.org/10.1016/j.inoche.2012.04.004
Brown DA, Kimari DM, Duzs-Moore AM, Budzichowski TA, Ho DM, Mandal SK. Reactions of dirhenium heptoxide with manganese(I) and rhenium(I) hydrido, alkoxo, methylcarbonato, carbonato-bridged, and methoxymethyl complexes. The X-ray structures of fac- (CO)3(dppp)MnOReO3 and fac-(CO)3(dppp)ReOReO3. J Organomet Chem 2002; 658(1-2): 88-93. https://doi.org/10.1016/S0022-328X(02)01632-7
Wang X, Tang S, Le S-Y, Lu R, Rader JS, Meyers C, et al. Aberrant expression of oncogenic and tumor-suppressive microRNAs in cervical cancer is required for cancer cell growth. PLoS One
Volinia S, Calin GA, Liu C-G, Ambs S, Cimmino A, Petrocca F, et al. A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA 2006; 103(7): 2257-61. https://doi.org/10.1073/pnas.0510565103
Lin SL, Chiang A, Chang D, Ying SY. Loss of mir-146a function in hormone-refractory prostate cancer. RNA 2008; 14(3): 417-24. https://doi.org/10.1261/rna.874808
Jazdzewski K, Murray EL, Franssila K, Jarzab B, Schoenberg DR, de la CA. Common SNP in pre-miR-146a decreasesmmature miR expression and predisposes to papillary thyroid carcinoma. Proc Natl Acad Sci USA 2008; 105(20): 7269-74. https://doi.org/10.1073/pnas.0802682105
Hurst DR, Edmonds MD, Scott GK, Benz CC, Vaidya KS, Welch DR. Breast cancer metastasis suppressor 1 up-regulates miR-146, which suppresses breast cancer metastasis. Cancer Res 2009; 69(4): 1279-83. https://doi.org/10.1158/0008-5472.CAN-08-3559
Bhaumik D, Scott GK, Schokrpur S, Patil CK, Campisi J, Benz CC. Expression of microRNA-146 suppresses NF-kappaB activity with reduction of metastatic potential in breast cancer cells. Oncogene 2008; 27(42): 5643-7. https://doi.org/10.1038/onc.2008.171
McClellan S, Slamecka J, Howze P, Thompson L, Finan M, Rocconi R, et al. mRNA detection in living cells: A next generation cancer stem cell identification technique. Methods 2015; 82: 47-54. https://doi.org/10.1016/j.ymeth.2015.04.022
Amatya VJ, Mawas AS, Kushitani K, Mohi El-Din MM, Takeshima Y. Differential microRNA expression profiling of mesothelioma and expression analysis of miR-1 and miR-214 in mesothelioma. Int J Oncol 2016; 48(4): 1599-607. https://doi.org/10.3892/ijo.2016.3358
Downloads
Published
Issue
Section
How to Cite
Similar Articles
- Teoh Han Pinn, Siti Fathiah Masre, Nadiah Abu, MiR-130b-3p Suppress the Migration, Proliferation and Chemosensitization of Hepatocellular Carcinoma Cells , Journal of Cancer Research Updates: Vol. 13 (2024)
- Hossein Mozdarani, Zainab Kouchak Mashkdouz, The Potent Regulatory Role of Circular RNAs in Breast Cancer Development, Diagnosis and Treatment: An Update , Journal of Cancer Research Updates: Vol. 11 (2022)
- Shaimaa A. Soliman, Laila A. Rashed, Mahmoud M. Said, Amina M. Medhat, Assessment of Gene Expression Level of ATP Binding Cassette G Member 2 (ABCG2) Transporter in Newly Diagnosed Breast Cancer Patients Receiving Adjuvant Chemotherapy , Journal of Cancer Research Updates: Vol. 8 No. 1 (2019)
- Parvin Mehdipour, Asaad Azarnezha, Eight-Hit Evolutionary Pattern in ATM Gene of a Breast Carcinoma Patient: A Personalized Approach , Journal of Cancer Research Updates: Vol. 10 (2021)
- Yujiro Naruse, Surface Functionalization of Gold Nanoparticles for Simultaneous Suppression of Cancer Stem Cells and Cancer Cells , Journal of Cancer Research Updates: Vol. 3 No. 3 (2014)
- Chao Lou, Rong Qiang, Hongmin Yan, Jie Li, High-Expression of PTEN and an Absence of PCNA in Osteoclast-Like Multinucleated Giant Cells of Giant Cell Tumors in Bone , Journal of Cancer Research Updates: Vol. 1 No. 1 (2012)
- Jiangting Hu, Ern Yu Tan, Leticia Campo, Russell Leek, Zainina Seman, Helen Turley, Domenico Delia, Alfredo Cesario, Kevin Gatter, Francesco Pezzella, TRAP1 is Involved in Cell Cycle Regulated by Retinoblastoma Susceptibility Gene (RB1) in Early Hypoxia and has Variable Expression Patterns in Human Tumors , Journal of Cancer Research Updates: Vol. 2 No. 3 (2013)
- Fatma F. Abdel Hamid, Mahmoud K. Singer, Mahmoud N. El-Rouby, Mahmoud M. Said, Reda H. Tabashy, Motawa E. El-Houseini, HepG2 Attenuation Induced by RNase A Modulates Gene Profiling and Immunophenotypic Characterization of Some Immune Cells Operating in Cancer Vaccine , Journal of Cancer Research Updates: Vol. 7 No. 1 (2018)
- Lin Gao, Qinhe Yang, Wenbin Zhou, Yong Dai, Jigang Wang, Li Fu, Pan Zhao, Jingy Huang, Malin Hong, Jinquan Xia, Chang Zou, RNA-Sequencing-Based lncRNA Biomarker Profiling on Triple Negative Breast Cancer , Journal of Cancer Research Updates: Vol. 10 (2021)
- Irene Ruiz, Freddy Fernando Durán, Carmen Sáez, Julián Sanz, Adela Pelayo, Fernando Moreno, Jorge Ruiz, Luis Ortega, Alejandro Pascual, HER2 Expression Discordance between Ductal Carcinoma In situ and Invasive Breast Carcinoma. How to Analyze Oncotype DX , Journal of Cancer Research Updates: Vol. 8 No. 1 (2019)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Christopher Krauss, Chelsey Aurelius, Kayla Johnston, Victoria Bartlette, Sherrita Gavin, Jazmine Cuffee, Satyendra Banerjee, Sarah Wiseniewsky, Santosh Mandal, Hirendra Nath Banerjee, An Investigation to Study the Role of Novel Rhenium Compounds on Endometrial Uterine Cancer Cell Lines , Journal of Cancer Research Updates: Vol. 9 No. 1 (2020)