Bringing Time into Molecular and Cellular Biology

• Karl Andersson

  Department of Radiation, Oncology and Radiation sciences, unit of Biomedical Radiation Sciences, Uppsala University, Sweden

 In conjunction with the defense of a doctoral thesis on the deciphering of complex protein interactions on living cells, six scientists shared their view on time in molecular and cellular biology. This brief review takes the form of a conference report and summarizes the contributions of the speakers and the defense. Opportunities and challenges for time resolved assays in molecular and cellular biology were vividly discussed during two days with a pan-European audience. Awareness of biological timeframes and understanding the temporal aspects were claimed critical for analytical applications in biology.

Chance B. The accelerated flow method for rapid reactions. J Franklin Inst 1940; 229: 737-66. http://dx.doi.org/10.1016/S0016-0032(40)90963-2

VanGuilder HD, Vrana KE, Freeman WM. Twenty-five years of quantitative PCR for gene expression analysis. BioTechniques 2008; 44(5): 619-26. http://www.ncbi.nlm.nih. gov/pubmed/18474036

Jönsson U, Fägerstam L, Ivarsson B, Johnsson B, Karlsson R, Lundh K, et al. Real -time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology. Biotechniques 1991; 11(5): 620-27. http://www. ncbi.nlm.nih.gov/pubmed/1804254

Björkelund H. Novel Methods for Analysis of Heterogeneous Protein-Cell Interactions: Resolving How the Epidermal Growth Factor Binds to Its Receptor. Doctoral thesis. In: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, Acta Universitatis Upsaliensis, ISSN 1651-6206; 854.

Zwang Y, Sas-Chen A, Drier Y, Shay T, Avraham R, Lauriola M, et al. Two Phases of Mitogenic Signaling Unveil Roles for p53 and EGR1 in Elimination of Inconsistent Growth Signals. Mol Cell 2011; 42(4): 524-35. http://www.ncbi.nlm.nih.gov/ pubmed/21596316 http://dx.doi.org/10.1016/j.molcel.2011.04.017

Mu Y, Sundar R, Thakur N, Ekman M, Kumar S, Yakymovych M, et al. TRAF6 ubiquitinates TGFβ type I receptor to promote its cleavage and nuclear translocation in cancer. Nat Commun 2011; 2: 330. http://www.ncbi.nlm.nih. gov/pubmed/21629263

Nilvebrant J, Kuku G, Björkelund H, Nestor M. Selection and in vitro characterization of human CD44v6-binding antibody fragments. Biotechnol Appl Biochem 2012; 59(5): 367-80. http://dx.doi.org/10.1002/bab.1033

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