pH Monitoring of Tumor Microenvironment and Low Volume of Urine in Experimental Rats

• Terezia Kiskova

  P.J. afárik University in Ko ice, Faculty of Sciences, Moyzesova 11, 040 01, Ko ice, Slovakia

• Steffekova Zuzana

  University of Veterinary Medicine and Pharmacy in Ko ice, Komenského 73, 041 81 Ko ice, Slovakia

• Karasova Martina

  University of Veterinary Medicine and Pharmacy in Ko ice, Komenského 73, 041 81 Ko ice, Slovakia

• Kokosova Natalia

  P.J. afárik University in Ko ice, Faculty of Sciences, Moyzesova 11, 040 01, Ko ice, Slovakia

 The pH monitoring of the tumor microenvironment in vivo seems to be in fact complicated and technically quite challenging nowadays. Also the strategy of measuring urine pH of a little amount is not fully solved. Thus, the aim of our study was to monitor pH of urine samples (< 0.1 ml) and of tumor microenvironment of anesthetized rats in a minimal invasive way. The small urine volumes of rats or mice make pH measurements difficult, as standard pH electrodes usually need a minimal volume of several milliliters to function. The manual micromanipulator together with a needle-type housed pH microsensor offers a simple and effective way to do so. Our results show that pH of urine and tumor microenvironment was lower in tumor bearing rats compared to healthy subjects. The unique technology of pH microsensors could be a promising way to monitor the pH in many experimental designs and clinical praxis.

Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell 2011; 144(5): 646-74. http://dx.doi.org/10.1016/j.cell.2011.02.013

Yang LV, Castellone RD, Dong L. Targeting tumor microenvironments for cancer prevention and therapy. Cancer Prev - From Mechanisms to Translational Benefits 2012; 3-40.

Robey IF, Baggett BK, Kirkpatrick ND, et al. Bicarbonate increases tumor pH and inhibits spontaneous metastases. Cancer Res 2009; 69: 2260-8. http://dx.doi.org/10.1158/0008-5472.CAN-07-5575

Wright ME, Michaud DS, Pietinen P, et al. Estimated urine pH and bladder cancer risk in a cohort of male smokers (Finland). Cancer Causes Control 2005; 16(9): 1117-23. http://dx.doi.org/10.1007/s10552-005-0348-9

www.presens.de

Danhier F, Feron O, Préat V. To exploit the tumor microenvironment: Passive and active tumor targeting of nanocarriers for anti-cancer drug delivery. J Control Release 2010; 148(2): 135-46. http://dx.doi.org/10.1016/j.jconrel.2010.08.027

Cairns R, Papandreou I, Denko N. Overcoming physiologic barriers to cancer treatment by molecularly targeting the tumor microenvironment. Mol Cancer Res 2006; 4(2): 61-70. http://dx.doi.org/10.1158/1541-7786.MCR-06-0002

Bailey KM, Wojtkowiak JW, Hashim AI, et al. Targeting the metabolic microenvironment of tumors. Adv Pharmacol 2012; 65: 63-107. http://dx.doi.org/10.1016/B978-0-12-397927-8.00004-X

Estrella V, Chen T, Lloyd M, et al. Acidity generated by the tumor microenvironment drives local invasion. Cancer Res 2013; 73(5): 1524-35. http://dx.doi.org/10.1158/0008-5472.CAN-12-2796

Alguacil J, Pfeiffer RM, Moore LE, et al. Measurement of urine pH for epidemiological studies on bladder cancer. Eur J Epidemiol 2007; 22(2): 91-8. http://dx.doi.org/10.1007/s10654-006-9101-2

www.socscistatistics.com

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