Sildenafil Induces Cell Cycle Arrest and Apoptosis in Human Colorectal Cancer HT-29 Cells

Wu-Ming Qin; Kun Wang; Jia-Rong Huang; Xiao-Long Mei; Zhi Shi

doi:10.6000/1929-2279.2018.07.02.4

Sildenafil Induces Cell Cycle Arrest and Apoptosis in Human Colorectal Cancer HT-29 Cells

Authors

Wu-Ming Qin Department of Cell Biology & Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China
Kun Wang Department of Cell Biology & Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China
Jia-Rong Huang Department of Cell Biology & Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China
Xiao-Long Mei Department of Cell Biology & Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China
Zhi Shi Department of Cell Biology & Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China

DOI:

https://doi.org/10.6000/1929-2279.2018.07.02.4

Keywords:

Sildenafil, Colorectal cancer, Cell Cycle, Apoptosis.

Abstract

Phosphodiesterase is an enzyme that degrades the phosphodiester bond in the second messenger molecules cAMP and cGMP, and regulates the localization, duration, and amplitude of cyclic nucleotide signaling within subcellular domains. Sildenafil is a potent and selective inhibitor of the type 5 cGMP-specific phosphodiesterase used clinically to treat erectile dysfunction and pulmonary arterial hypertension. In this study, we examined the effect of sildenafil on human colorectal cancer HT-29 cells. Our data showed that sildenafil induced cell cycle arrest and apoptosis, and cotreatment with a ROS scavenger N-acetyl-L-cysteine partially reversed cell apoptosis caused by sildenafil in human colorectal cancer HT-29 cells. Overall, our study suggests that sildenafil appears to be a promising new treatment option for colorectal cancer.

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