P53 Codon 72 and Endometrium Cancer


  • F. Gloria-Bottini Department of Biomedicine and Prevention, University of Rome, Tor Vergata, Rome, Italy
  • M. Nicotra Department of Gynaecologic, Obstetric and Urologic Sciences, University of Rome La Sapienza, Rome, Italy
  • C. Spina Department of Biomedicine and Prevention, University of Rome, Tor Vergata, Rome, Italy
  • P.L. Benedetti-Panici Department of Gynaecologic, Obstetric and Urologic Sciences, University of Rome La Sapienza, Rome, Italy
  • P. Saccucci Department of Biomedicine and Prevention, University of Rome, Tor Vergata, Rome, Italy
  • A. Neri Department of Biomedicine and Prevention, University of Rome, Tor Vergata, Rome, Italy
  • A. Magrini Department of Biomedicine and Prevention, University of Rome, Tor Vergata, Rome, Italy
  • E. Bottini Department of Biomedicine and Prevention, University of Rome, Tor Vergata, Rome, Italy




Endometrium cancer, p53 codon 72, ADA1, ADA6, PTPN22.


 Background: The possible role p53 codon 72 in endometrium cancer has been investigated in several human populations: a positive association with the Pro variant has been observed in Asiatic but not in Caucasian populations. We reasoned that polymorphisms associated with endometrium cancer may interact with p53 codon 72 influencing the degree of association between this polymorphism and cancer.

Methods: Sixty nine women admitted to the hospital for endometrium cancer and 473 healthy subjects were studied in the White population of Rome. Verbal consent was obtained from these subjects to participate to the study that was approved by the Department. P53 codon 72, ADA1, ADA6 and PTPN22 genotypes were determined by DNA analysis Statistical analysis were performed by using commercial software (SPSS).

Results: The joint genotype carrying the *Pro allele of p53 codon 72and the ADA1*2 allele, the joint genotype carrying the *Pro allele and ADA6 *1 allele and the joint genotype carrying the *Pro allele and *C/*C genotype of PTPN22 show a proportion greater in cancer than in controls. The proportion of *Pro allele carriers in endometrium cancer shows a positive correlation (p=0.019) with the number of genetic factors considered i.e. ADA1,ADA6, PTPN22.

Conclusion: Our data suggest that the strength of association between the disease and p53 codon 72 depends on other genetic factors. Thus the different patterns of association between p53 codon 72 and endometrium cancer observed among human populations could be at least in part related to differences in allelic frequencies of these genetic factors.


Matlashewski GJ, Tuck S, Pim D, Lamb P, Schneider J, Crawford LV. Primary structure polymorphism at amino acid residue 72 of human p53. Mol Cell Biol 1987; 7: 961-3. http://dx.doi.org/10.1128/MCB.7.2.961

Roh JW, Kim JW, Park NH, Song YS, Park IA, Park SY, Kang SB, Lee HP. p53 and p21 genetic polymorphisms and susceptibility to endometrial cancer. Gynecol Oncol 2004; 93: 499-505. http://dx.doi.org/10.1016/j.ygyno.2004.02.005

Zubor P, Stanclova A, Kajo K, Hatok J, Klobusiakova D, Visnovsky J, Danko J. The p53 codon 72 exon 4 BstUI polymorphism and endometrial cancer in Caucasian women. Oncology 2009; 76: 173-83. http://dx.doi.org/10.1159/000201570

Gu Y, Zhou X, Zhang SL. Meta-analysis of an association of codon 72 polymorphisms of the p53 gene with increased endometrial cancer risk. Genet Mol Res 2011; 10: 3609-19. doi: 10.4238/2011

Tang W, He X, Chan Y, Luo Y. Lack of association between p53 codon 72 polymorphism and endometrial cancer: a meta-analysis. Cancer Epidemiol 2012; 36: e153-7. http://dx.doi.org/10.1016/j.canep.2011.12.010

Kafshdooz T, Tabrizi AD, Mohaddes Ardabili SM, Kafshdooz L, Ghojazadeh M, Gharesouran J, Abdii A, Alizadeh H. Polymorphism of p53 gene codon 72 in endometrial cancer: correlation with tumor grade and histological type. Asian Pac J Cancer Prev 2014; 15: 9603-6. http://dx.doi.org/10.7314/APJCP.2014.15.22.9603

Yoneda T, Kuboyama A, Kato K, Ohgami T, Okamoto K, Saito T, Wake N. Association of MDM2 SNP309 and TP53 Arg72Pro polymorphisms with risk of endometrial cancer. Oncol Rep 2013; 30: 25-34. http://dx.doi.org/10.3892/or.2013.2433

Gloria-Bottini F, Nicotra M, Benedetti-Panici PL, Pietropolli A, Saccucci P, Ambrosi S, Bottini E, Magrini A. Genetic Variability within ADA Gene and Endometrium Cancer. Journal of Genetic Syndromes and Gene Therapy, ISSN 2157-7412.

Wiginton DA, Kaplan DJ, States JC, Akeson AL, Perme CM, Bilyk IJ, Vaughn AJ, Lattier DL, Hutton JJ. Complete sequence and structure of the gene for human adenosine deaminase. Biochemistry 1986; 25: 8234-44. http://dx.doi.org/10.1021/bi00373a017

Tzall S, Ellenbogen A, Eng F, Hirschhorn R.Identification and characterization of nine RFLPs at the adenosine deaminase (ADA) locus. Am J Hum Genet 1989; 44: 864-75.

Londos C, Honnor RC, Dhillon GS. cAMP-dependent protein kinase and lipolysis in rat adipocytes. III. Multiple modes of insulin regulation of lipolysis and regulation of insulin responses by adenylate cyclase regulators. J Biol Chem 1985; 260: 15139-45.

Ohisalo J, Strandberg H, Kostiainen E, Kuusi T, Ehnholm C. Stimulation of lipoprotein lipase activity of rat adipose tissue and post-heparin plasma by N6-(phenylisopropyl)adenosine. FEBS Lett 1981; 132: 121-3. http://dx.doi.org/10.1016/0014-5793(81)80442-5

Vannucci SJ, Nishimura H, Satoh S, Cushman SW, Holman GD, Simpson IA. Cell surface accessibility of GLUT4 glucose transporters in insulin-stimulated rat adipose cells. Modulation by isoprenaline and adenosine. Biochem J 1992; 288(Pt 1): 325-30. http://dx.doi.org/10.1042/bj2880325

Challis RA, Budohoski L, McManus B, Newsholme EA. Effects of an adenosine-receptor antagonist on insulin-resistance in soleus muscle from obese Zucker rats. Biochem J 1984; 221: 915-7. http://dx.doi.org/10.1042/bj2210915

Challis RA, Richards SJ, Budohoski L. Characterization of adenosine receptor modulating insulin action In skeletal Eur J Pharmacol 1992; 226: 121-128. http://dx.doi.org/10.1016/0922-4106(92)90172-R

Kameoka J, Tanaka T, Nojima Y, Schlossman SF, Morimoto C. Direct association of adenosine deaminase with a T cell activation antigen, CD26. Science 1993; 261(5120): 466-9. http://dx.doi.org/10.1126/science.8101391

Dong RP, Kameoka J, Hegen M, Tanaka T, Xu Y, Schlossman SF, Morimoto C. Characterization of adenosine deaminase binding to human CD26 on T cells and its biologic role in immune response. J Immunol 1996; 156: 1349-55.

Franco R, Casadó V, Ciruela F, Saura C, Mallol J, Canela EI, Lluis C. Cell surface adenosine deaminase: much more than an ectoenzyme. Prog Neurobiol 1997; 52: 283-94. http://dx.doi.org/10.1016/S0301-0082(97)00013-0

Spencer N, Hopkinson D, Harris H. Adenosine deaminase polymorphism in man. Ann Hum Genet 1968; 32: 9-14. http://dx.doi.org/10.1111/j.1469-1809.1968.tb00044.x

Bottini N, Musumeci L, Alonso A, Rahmouni S, Nika K, Rostamkhani M, MacMurray J, Meloni GF, Lucarelli P, Pellecchia M, Eisenbarth GS, Comings D, Mustelin T. A functional variant of lymphoid tyrosine phosphatase is associated with type I diabetes. Nat Genet 2004; 36: 337-8. http://dx.doi.org/10.1038/ng1323

Ammendola M, Gloria-Bottini F, Sesti F, Piccione E, Bottini E. Association of p53 codon 72 polymorphism with endometriosis. Fertil Steril 2008; 90: 406-8. http://dx.doi.org/10.1016/j.fertnstert.2007.06.051

Sebastiani GD, Bottini N, Greco E, Saccucci P, Canu G, Lucarelli P, Gloria-Bottini F, Fontana L. A study of Adenosine-Deaminase genetic polymorphism in rheumatoid arthritis. Int J Immunopathol Pharmacol 2010; 23: 791-795.

Gloria-Bottini F, Ammendola M, Saccucci P, Pietropolli A, Magrini A, Bottini E. The association of PTPN22 polymorphism with endometriosis: effect of genetic and clinical factors. Eur J Obstet Gynecol Reprod Biol 2013; 169: 60-3. http://dx.doi.org/10.1016/j.ejogrb.2013.01.014

SPSS/PC+ version 5,Chicago: SPSS, Inc 1992.

Hoskin DW, Mader JS, Furlong SJ, Conrad DM, Blay J.Inhibition of T cell and natural killer cell function by adenosine and its contribution to immune evasion by tumor cells (Review). Int J Oncol 2008; 32: 527-35. http://dx.doi.org/10.3892/ijo.32.3.527




How to Cite

F. Gloria-Bottini, M. Nicotra, C. Spina, P.L. Benedetti-Panici, P. Saccucci, A. Neri, A. Magrini, & E. Bottini. (2015). P53 Codon 72 and Endometrium Cancer. Journal of Cancer Research Updates, 4(4),  149–152. https://doi.org/10.6000/1929-2279.2015.04.04.2