In Vitro Evaluation of the Antiinflammatory and Anticancer Activities of Compounds from the Plants Casearia sylvestris and Zanthoxylum monophyllum

Omar A. Dupuy L.; José A. Bonilla V.; Renato Murillo M.; Peter Taylor; María J. Abad; Lorena I. González P.; Olmedo Otero; Johanna Juliao A.

doi:10.6000/1929-2279.2015.04.03.2

Authors

Omar A. Dupuy L. Instituto de Investigaciones en Biotecnología y Ciencias Biomédicas, Escuela de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panamá, Panamá
José A. Bonilla V. Centro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, San José, Costa Rica
Renato Murillo M. Escuela de Química y CIPRONA, Universidad de Costa Rica, San José, Costa Rica
Peter Taylor Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Apartado 21827, Caracas 1020-A, Venezuela
María J. Abad Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Apartado 21827, Caracas 1020-A, Venezuela
Lorena I. González P. Instituto de Investigaciones en Biotecnología y Ciencias Biomédicas, Escuela de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panamá, Panamá
Olmedo Otero Instituto de Investigaciones en Biotecnología y Ciencias Biomédicas, Escuela de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panamá, Panamá
Johanna Juliao A. Instituto de Investigaciones en Biotecnología y Ciencias Biomédicas, Escuela de Biotecnología, Facultad de Ciencias de la Salud, Universidad Latina de Panamá, Panamá, Panamá

DOI:

https://doi.org/10.6000/1929-2279.2015.04.03.2

Keywords:

4´-O-methyl-gallocatechin, columbianatin, casearin G, peripheral blood mononuclear cells, B16/BL6 melanoma, COS-7.

Abstract

The anthocyanins, coumarins, and casearins are interesting compounds due to their chemical and biological properties. In the present study, the in vitro effect of 4 ́-O-methyl-gallocatechin (an anthocyanin), columbianatin (a coumarin), and casearin G on the mitogenic response and phagocytic activity from whole blood cells, was evaluated. In addition, the cytotoxic effect of these compounds on the cell lines B16/BL6 (murine melanoma) and COS-7 (kidney fibroblasts transfected with SV40 virus) was measured by a colorimetric assay (MTS/PMS, IC50: inhibitory concentration) and the sulphorhodamine B assay (GI50: growth inhibition, TGI: total growth inhibition, LC50: lethal concentration). The results show that 4 ́-O-methyl-gallocatechin and columbianatin reduced lymphoproliferation. Columbianatin reduced both the phagocytic index and the percentage of phagocytic monocytes/macrophages. Casearin G showed both cytotoxic (IC50, LC50) and cytostatic (GI50) effects against the tumor cells, B16/BL6 (IC50 = 30.8 μM; GI50 = 12.4 μM; LC50 = 34.7 μM) and COS-7 (IC50 = 137.3 μM; GI50 = 3.8 μM; LC50 = 29.9 μM). In conclusion, 4 ́-O-methyl-gallocatechin and columbianatin showed immunosuppressive properties in vitro while casearin G was the most cytotoxic.

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