Bufalin Induces Apoptosis of MDA-MB-231 Cell Through Activation of JNK/p53 Pathway


  • Jian-Yang Hu College of Pharmacy, Jinan University, Guangzhou 510632, P.R., China
  • Min-Feng Chen College of Pharmacy, Jinan University, Guangzhou 510632, P.R., China
  • Xue-Ping Lei College of Pharmacy, Jinan University, Guangzhou 510632, P.R., China
  • Zhen-Jian Zhuo College of Pharmacy, Jinan University, Guangzhou 510632, P.R., China
  • Hai-Yan Sun Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
  • Zhe-Sheng Chen College of Pharmacy and Health Sciences, St. John's University, Queens, New York, USA
  • Zhi Shi College of Life Science, Jinan University, Guangzhou 510632, P.R., China
  • Dong-Mei Zhang College of Pharmacy, Jinan University, Guangzhou 510632, P.R., China
  • Wen-Cai Ye College of Pharmacy, Jinan University, Guangzhou 510632, P.R., China




Apoptosis, JNK, p53, Bcl-2, MDA-MB-231 cell.


 Cinobufacini has been widely used at oncology clinics in China to treat many kinds of cancers and bufalin is one active compound of it, but the anti-cancer effect and the underlying mechanisms of bufalin on breast cancer cells are still unclear. Our study demonstrated that bufalin could effectively decrease the viability of MDA-MB-231 cells with IC50 values of 152.2 ± 8.0 nM for 24 h and 22.4 ± 2.3 nM for 48 h, respectively. Further study showed that bufalin could trigger MDA-MB-231 cells to undergo apoptosis with up-regulating protein expression of p-JNK/JNK, p53, p-ERK/ERK, Puma and down-regulating protein expression of Bcl-2, supporting the possible application of bufalin to breast cancer treatment.


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How to Cite

Jian-Yang Hu, Min-Feng Chen, Xue-Ping Lei, Zhen-Jian Zhuo, Hai-Yan Sun, Zhe-Sheng Chen, Zhi Shi, Dong-Mei Zhang, & Wen-Cai Ye. (2015). Bufalin Induces Apoptosis of MDA-MB-231 Cell Through Activation of JNK/p53 Pathway. Journal of Cancer Research Updates, 4(2),  47–53. https://doi.org/10.6000/1929-2279.2015.04.02.1