A HPLC-UV Method for the Quantification of Regorafenib in Tumor
DOI:
https://doi.org/10.30683/1929-2279.2020.09.01Keywords:
Regorafenib, HPLC-UV, tumor.Abstract
Regorafenib has been approved for the treatment of colorectal cancer, gastrointestinal stromal tumor and hepatocellular carcinoma. High-performance liquid chromatography (HPLC) was developed and validated for determination of regorafenib in xenograft tumors. After protein precipitation with acetonitrile, regorafenib were separated using gradient elution (C18 Ultrabase column). Quantification was performed at 262 nm. Calibration curves were linear over the range 48.8-50000 ng/ml. The assay was applied to the determination of the drug in the tumor of nude mice receiving regorafenib 50 mg orally, and could be useful for therapeutic drug monitoring of regorafenib in routine clinical practice.
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