Non-Invasive Real-Time Monitoring and Label-Free Sensing of Hydrogen Peroxide in Human Brain Cancer Cells Through Raman Spectroscopy
Keywords:Raman spectroscopy, human glioblastoma, hydrogen peroxide, biomarkers, cellular metabolic activity
We present evidence that real-time optical monitoring of human brain cancer cells through forward Raman spectroscopy can be used as a highly sensitive non-invasive label-free tool in acquiring real-time information on the cellular metabolic activity through the concentration levels of selective biomarkers. In this investigation, we quantified the concentration level of a natural by-product of cellular respiration, hydrogen peroxide (H2O2), through its characteristic Raman signature due to the transitions of O-O vibration energy level, resulting in a sharp Raman scatter frequency shift around 880cm-1. The experimental findings revealed that 3.75´106 cells to have produced a substantial amount (3%) of H2O2.
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