A Novel Method to Radiolabel Stealth Liposome through 1,2- dimyristoyl-sn-glycero-3-phosphoethanolamine-N-DTPA with 99mTc and Biological Evaluation

Authors

  • Mirel Cabrera Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay
  • Alejandra Medrano Departamento de Ciencia y Tecnología de Alimentos, Facultad de Química, Universidad de la República. General Flores 2124, 11800, Montevideo, Uruguay
  • Nicole Lecot Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay
  • Marcelo Fernandez Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay
  • Maria Moreno Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República. Av.Alfredo Navarro 3051, 11600, Montevideo, Uruguay
  • Jose A Chabalgoity Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la República. Av.Alfredo Navarro 3051, 11600, Montevideo, Uruguay
  • Juan Pablo Gambini Centro de Medicina Nuclear, Hospital de Clínicas “Dr. Manuel Quintela”, Facultad de Medicina, Universidad de la República. Av. Italia s/n, 11600, Montevideo, Uruguay
  • Omar Alonso Centro de Medicina Nuclear, Hospital de Clínicas “Dr. Manuel Quintela”, Facultad de Medicina, Universidad de la República. Av. Italia s/n, 11600, Montevideo, Uruguay
  • Henia Balter Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay
  • Pablo Cabral Arera de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República. Mataojo 2055, 11400 Montevideo, Uruguay

DOI:

https://doi.org/10.6000/1927-7229.2013.02.01.1

Keywords:

Liposome, nanomedicine, diagnostic oncology

Abstract

Purpose: To study surface technetium labeling of stealth DTPA-Liposomeand to evaluate its potential as a molecular imaging tracer for both normal and melanoma-bearing mice.

The radiolabeling yield of liposomes was greater than 90%and showed good chemical and biological stability. Biodistribution studies in normal mice showed blood clearance with hepatic and renal depuration. Melanoma-bearing mice showed a similar pattern of biodistribution with high tumor uptake allowing tumor imaging.

The developed method of surface radiolabeled DTPA-PEG-Liposomes with 99mTc was effective and stable in vivo.

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Published

2013-01-14

How to Cite

Mirel Cabrera, Alejandra Medrano, Nicole Lecot, Marcelo Fernandez, Maria Moreno, Jose A Chabalgoity, Juan Pablo Gambini, Omar Alonso, Henia Balter, & Pablo Cabral. (2013). A Novel Method to Radiolabel Stealth Liposome through 1,2- dimyristoyl-sn-glycero-3-phosphoethanolamine-N-DTPA with 99mTc and Biological Evaluation . Journal of Analytical Oncology, 2(1), 1–9. https://doi.org/10.6000/1927-7229.2013.02.01.1

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